                          ˿µϣ20081012գ

08.10.11ûи
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08.10.12, ϴѧޱͬBao Tong Cui)ڼȫĳϮ⿯
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                ѧʷ˭˰̲

                           ӡ

    106գѧˡ͸˿Ͷŵ
ְ̲뷢ͷ״ĵ¹ҽ¡ɭ
ŵѧҽѧϢѧ޲ءڽ
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꣬ڿ˹ҽѧԺ֮ǰʵ
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1983520ճѧ־ϱ֡

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HIV1986꣬ڷְ̲˹ҽ
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˼ȴܻר¶ֲ֮ȡϢ˵10꣬
ýĽ룬˹ٷͽۡʵҵ
棬صĺгŰ칫Ϊ
ص谭˰̲оĽչΪΪ


2008.10.8

ù۲챨2008.10.13

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ĿƼ

ߣˡ

ҡҡԡרҡݲμĳؿί֯ĿƼ
顣뵽¹̷ۡҲùҿƼٶԱȸոŵ
ѡɵп

ǵڿίУίҪ쵼ȫᣬר
ԱʮλƼкüأ걨Ȼʽ
飬ٺר󣬾ǸɵĻ滹йʾȹ̡˵
רȨԵһء

ר󻹷һרҵ󣬵ڶۺרҵ
ίԱҪǴĳרҵļԱǸλŵ쵼Ҳרҵ
Աɡ֪ôʲôǵļһרίԱᣬҲ
Ҫ׺һ°ɡۺίԱҪɸ쵼ɡӡר󡱵
󡱣Ҳ˵רҡӡϰѹأ쵼ڡ塱ϰա

ѡ׼ģȻ׼װϣӡֽϵġ
˵ɹˮƽǹȻǹȣԡ״
ֶСѶȡȡЧЧ桱
Ӧƹֵָ꣬ÿָ궼ֽ⣬ϸĴϸ

ǡרҡĹĶЩϣȻ飬ÿɹ
֡Ƽ漰רҵټ걨Ϻܶ࣬
鳤ÿԼϤ򡣶Բıɹôأǻ
ʱ˷ο֣ԼŰɡ

鳤㼯ĿıȼҲ˵ص
һȽ⡣Ȼдֲϻдǩֻ
ǩóԺúд٣ٷٴΪ͡ǡרҡ¾
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Ǻܡѧġָ꣬ҲǺܡϽӴ
λǧҪΪƼ͸ĳ˵ģҪϡġ

¹̷ۡơƼɹҲƵı
һдúƯˮƽǹȾǹȣĸר
˵֡˼ǿ˿Ƽɹģôרǩֵģ
±桢ⱨ桢ûϵȵȣ֤һһѵģĸ
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˵̷谷ļѣЧ桱ҵ̷һʮڣ
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֪ѧƼʼҿѧԺʱֻģ󣬲ûʲô
ָ꣬ҲҪࡢ֮ġ쵼ѹءôŵô
Ȩأ

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裺Ƽشҩơ

ߣԹZ

شҩơڶڿʼˣһѾϣƺȫҩ
ҵнһδԾ״̬˶д飬֪ж
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ĳҩƷʳƷ춨ǣͷ˹ڵĿĹͷ
˳ˣЩǮͲ֪ôˣҲ֪ͨʲôˣ
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ֵĽǷҹʡ˵ЩԵ˶
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Ŀ𣿾ɸѡؼƽ̨ҩٴǰҩЧѧۼƽ̨
Ŀ׫дν쵼СԱܲҪ
˹ƽ

裡

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УƾԴ?

ߣЦЦ

͸ʡίУ챾ơоѧ뵳Уղʿо
һһֱ۷׷׷з

йУڰ䲼ʵʩУǰУѧ
ͬȹйشɾȥӦΪУѧͬȹ
ʹִõһ׶ԵĽϤȫø
Уƾ߳320

Ҳһְͬѧһܴˡǰͨ
ԻȡҳϵרѧΪ˽ȥ뵳Уĺڰ࣬
뵳Уƾֵ˵ְֵá



Уƾ𣿵ȻáУƾǸ˵ְש͵
ʯٵĿһԣԼǮǮҳˣͿûʻʵ
ƣоʲôƾڹٳһܡֻҪڵ٣
ƾЧģžûϿˣйɫƾ



УѧϰԿɣڵĹ,һ·ն,һ˳,
УޱȵһѧĹƾ,ְ
ƺͿԱʱƽƽ,,ѷٲ㵳УѧԱġ
ˣ׷УѧΪһ˻ȡѧĽݾʵеĵУѧ
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ûжֵۣۡǣҲֻѵԱɲ
һѧרѧУ199911ʩеġл񹲺͹
ȽеĵʮߵȽʵʩѧߵȽ˵ú
Ǹ˵ѧԺ㲻һָߵԺУأӦҲһֻ
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ôиҵѵţë˵ѵ㲻㣬
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ΪҽΨһͳ˽ı׼ԵУ
Գϵ͹ϵ࿹⣬ɽͷԶ׼Էƾ⻹
漰ƽ˵һڹϵ֮רѵҲѧ
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ϵƾᲢˡǾİɣٳµ

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Ҵ˳һʱǹһֳ衣

ϵУƾѾá

198611£ҽίԱᡶ뵳УĺѧԺ
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С

19957£ҽίԱᡶ굳УѧӦͬڹѧ
ĸָУѧӦͬڹϵеѧΪ
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˵̬Ⱥȷˡ

ǲǵ͹ײأ˵ˣһңҪ
ǵһ֣ĹȣֵļײһҲ
ֵ֡ǵУôҲǮأͰĵԱѵ
ˣʲôѧ࣬ƣоʿ࣬ⲻ
ǮҪ㺦ȫļѧԱأ

·

˵ϳиУ䷢ƾ㲻ȡ¼ַʽ


԰ΪʱҪĻ,Ҫ֪ܵдĳ
ĳĳУѧϰ;Ҳдϡְоְʿ,δעڵУ
ѧϰ,Ա,;дϡڵУ(ĳĳĳĳ
);ڵУѧϰ(һߴȥ);ҪдϡڵУѵ(Ī);
һֿҪʾհ׻һʴ,˵ǡμӹĳְҵѵ(
Ҳһְҵ)

Уƾйɫһֵֽ˼

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йڡӢҽѧ־Ϸ¡и

ߣPlymouth

ءᵽڡӢҽѧ־Ϸһƪ

Zhenglin Yang
From the Sichuan Academy of Medical Sciences and Sichuan 
Provincial People's Hospital, Chengdu, China 
Toll-like Receptor 3 and Geographic Atrophy in Age-Related Macular 
Degeneration
Volume 359:1456-1463 October 2, 2008 Number 14

鿴ԭģhttp://content.nejm.org/cgi/reprint/359/14/1456.pdf

֣ԭһ˫ݣ ᵽĴԱҲ
University of Utah School of Medicine, Salt Lake City һԱͨѶ
֮һKang Zhang, M.D., Ph.D Ҳ˫ݣһInstitute of 
Molecular Medicine, Peking University, Beijing, China (K.Z.). 
Shiley Eye Center, University of California at San Diego, San Diego, 
CA߾˸ӦΪɣҹʵҹϵ
Ϊʲôö߶Ϊ˫ݣ ϸο
http://forum.e2002.com/viewthread.php?tid=133575.

ԭΪѧΪ

(XYS20081012)

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ϴѧޱͬBao Tong Cui)ڼȫĳϮ⿯

ysxin

ײʱ֣ⷢϴѧޱͬBao Tong Cui)ڼ
ȫĳϮ⿯ģȤĿԱȶһ£

Observer-based passive control of linear time-delay systems with 
parametric uncertainty
Chaos, Solitons & Fractals, Volume 32, Issue 1, April 2007, Pages 
160-167
Bao Tong Cui, Mingang Hua

Robust H observer design of linear time-delay systems with 
parametric uncertainty
Systems & Control Letters, Volume 42, Issue 4, 6 April 2001, Pages 
303-312
Zidong Wang, Biao Huang, H. Unbehauen

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ж

zhenhan08

¼з˵רҿҩƷɫǳһел
ǣеķڵϴֹİжעҺܵȾһ
²ǿĲԭ΢˵ѶŵĴעҺ
˶飬ʾͼƷûмǿлũҩ
ũҩ֬ũҩݼȳɷ֡

֪רΪʲôһѴעҺԭǵϸ΢
ȾũҩȾԭȥѵ⡢Ⱦ⣬ҩעҺ
⣿ԭ򣬹ǰߣͷһҵ͹ˣںζ
һҵ

ҩȾ˳ǿ²΢ֻ˶󲿷ʹûиȾ
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Ʃũҩÿ˳˶жӦ̶ֻ˿ͦ
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Щ˶ĳﷴӦǿңЩ޷ӦʹעҺ
ٴǺϡ󲿷˿ܴӽ壬ٲ˻ᷢ
ӦٲǿҵĹӦμ˵ͬʱ
3 ˣʼ͵ǷˡɼⲢжӦǹӦ

ҪǣֱӽѪҺĴעҺ溬мʮֻϰֵ
ѧɷִڡ֪ѲͬεҩȥгɷַǷÿһֳ
ֶڻߺͬʮֻϰֳɷһβû֪
ģһ׵Ҳδ֪ġ˹Ӧ
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ǿԶֱ֪ӽѪܵҩ˲ãԼ
оԭѣֻýԭڲƷȾˡǲƷ
ǻԹΪ⣬ʳ¼ķ

˳ҩԶԷӦͼԹӦȴȻΪ䳪޸裬
ε磡

˵֪ǷרҵԴרָ 

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СľˮۣңԶĹŴǺȽʶ
ǳձʶҽѧΪ۵ġս
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У۶ҽָͻ

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ĹŴ۵ĸ߶ȣձָһУȫġ
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˵ҽˣ͵˵еˡôˣĪˮ
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֡ҽĻβ

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໰ﶼҽĴʻйϵͨ׺УĿϣ
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СĿʹʹȵȡ

ҽϻĻβ

ֱ磬˵Ŀڴҽ˵ǽСһ
Ⱦɵġǣйˣҽǰգ˶˵ǡϻ
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𡱣Ǿ͸֪Ĳ֢ˡ

ҽۣǡϻ𡱣ҽ硢ʪ
С֮һҽ˵ǡ䲡ǡȾ
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˵ǳϰߣû˲⡣

ġĻβ

ֱЩסҽҽԺһаҽȫ
ʽĴҽ˻кԺصңǣеĻҪ
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ﲻšеĲҿȡҿյ˾ءӡ
ӡڲӷ棬ȻԵҽûˣҽĻ
кܶܶࡣ

Ļкܶ࣬һһо١֮й˴ճβ
ʶϰߵ֣ǱպҽĻɵĸҩʹҽ˵
£Ļǳι̵Ĳдǵкϣ
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ľٶı֣ͶΪҲåõ
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ҽĻĴβڽµ׾ӱݣ1˵ҽãԼ
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ġҽСȡͬʱҪҽ߳š4ӣ
ҽĻβͣƭȡǮƣ󷢺ƣ5ҽĻβʹ
ʹһЩ֪ˣԸҽƭƭȡǮƣϵƭԸ
6ӿðҽҽåҽʿɫ
壬ϧҥ¡ݺ

ҽĻĴβͣйĻ׸׸վҪġ
ҽĻ׸ȻҪܳʱ䣬ǣֻҪԡҽʵ
γɹ˵ĹʶֻҪʶʤ̭ıȻôĻ
βͶΣþͻᱻ޺Ĳ֣Գڴȥ

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УɯʿǺ͵Ҹ˹εŵѧѽ

ߣſڿ

տһţǣ绪ѧŵΪȻ

ſڿӵ˵

ҾϵҷΪ֣һǵùŵѧģһ
ûùŵѧģԸμӺһ顣ΪһΰҺܶ
ܶ࣬Գŵѧһ顣ɯʿǡж˹̩Ҹ˹ˡ
£ûùԵýҲûôֵע⣬Ҿòظ
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Ҫſڿ󵼺ҲҪԲѱ˵
ġ

ԭĲ鿴ӣhttp://news.sohu.com/20081011/n259963788.shtml

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й60˹ϳţȵоûлŵԭ

½ (Jiang John Chen)
Ǵѧ(University of Georgia)ϸѧϵ(Department of 
Cellular Biology) ʿ

һһȵŵİ佱գйѧٴŵԵ
ڲվ϶ᵽйŵù
źĻ60˹ϳţȵصоɹûõŵԭ
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߲ءɹVincent du Vigneaud1955ŵѧ
ŵҳwww.nobelprize.orgҲȷָýԭ
for his work on biochemically important sulphur compounds, 
especially for the first synthesis of a polypeptide hormone
Vincent du Vigneaudȣйѧںϳţȵصķϲûб
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ҳҲָԭǡfor his development of methodology for 
chemical synthesis on a solid matrixɴ˿ɼŵصǷ
ϵͻƺʹ¡

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ԵĶĻߵס֮ǰţȵغʹ߲طС֮
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Եͻƺʹ£ܹشԶӰоɹ

(XYS20081012)

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ҲΪ2008ŵѧ´޵ƽ
ʦġӫﻯѧҡ֮

ߣ֮ǽ

ʦġӫﻯѧҡ֮󣬶
˹֮һ´ޣOsamu Shimomuraоеǳظп
ʦûᵽһЩʵݽҳйصϣ
´޵¼ʣʹƽٷḻһ㡣Ըⲻǻ
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1ѧھƳѧУڵϸУ
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оһμˮĸĹԱоߴʮ֮ڡ

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ش˵ΪʲôбҪصسΪأձҲԾס
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1http://www.asahi.com/science/update/1008/TKY200810080259_01.html
2http://www.yomiuri.co.jp/science/news/20081008-OYT1T00560.htm

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йѧ߶ҵܹŵ

ߣŽả
2008-10-12ձ

й˴Ϊʲôܹڰ˻ϻýƵһΪ˻һ
ҵɳһɫרҵ˶ԱйѧΪʲôҲû
һöŵĽƣΪѧоһרҵǵѧ߶
ҵѡ̫࣬һ㶼˵ЩѧƵĿжһ֧ҵ
顣

ڻ÷¡ѧиͳݵĽǰҪһΡ
ⷢ఩ϡʲסԺ֮ǰӦһ
ݽݽˡӡȴһ仰
֪ҳɹؾκһ9㵽Ұ칫
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ʵϣŷѧ߶ԣ9㻹ڹǸ
ǵĹ״̬뵽һʡѵ֪й˶Ա
ȡھؾ

йѧ߶飬ҲҪʣ֮Ϊҵѡ֡ѧ
Ҳҵ˶Աһͬʱһְҵǵǡ˵
ҵģΪԣǵҪ˼ѧоϡ磬
9ǿҲææǺѧо޹ϵ顣

ôǵҪ˼ŵȥأ

ʵܼ򵥣һ١ڶǷơǼַơ
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ϵ߼ȫͨģ뵽ǮҿԲ
㣬ؼҪʲôɹ

磬ԱͬĿгɹһ˴ʾ
ģһǺٵľѻõġڶӦõõνΪǮ
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(XYS20081012)

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ڹݴѧѧԺԺڸڵһЩ»ظĻظ

ߣı

̳ת˿ݴѧѧԺԺڸ
һЩ¡XYS20080602һλYhong888000һѡԵ
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1.	Ƕ֪͹󹤺ϲҲ֪ҵϵѧԺϲ
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ͣһƫеһ㣬һƫһ㣬Ըҵ⣬Ҫôеѧ
ԺϲҪôѧԺϲҴû˵йĹҵר
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ҵרҵĻظУҲ֪еѧԺĹҵרҵ˻
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ѧԺϲˡȻ˵ãȻȷȻ211ѧĴ¡

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<http://210.40.32.30/netpart/xueyuan/content.asp?id=278&dpt=tjxy&Flag=1>

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ѧо£оֱе06Ļеƺۣ08
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еѧԺѧԺϲѧԺԺԵҵרҵ˶
ȴлеѧԺؼ㲿ƵоֲԴˣ 
<http://www.gzu521.com/campus/article/postgraduate/200708/156783.htm>

7.	ԭѾˣӲԭ渽ʵҲ
ѵһҪÿһӶﲻɣ˻`ӣϵλ
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ְƣͬʱҲͻڹҵĹλ˭Ҳ
ȥ飬鵽Ҳ˵һ󡣽ְǱ󣬹ҵѧԺԺ
һ仰ож󣬶ҶǱͷϣΪȫ˵
һ
<http://www.hbmes.com/Pages/NewsContent.aspx?InfoID=44>

Ҳ⣬ѧԺĹҵרҵǹΪ211ѧĴ֮٣
ϧѧĽǿרǣԼһڱȽ
ªŵˣһЩ´죬·±Ц
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Դ͵ĵ̻ظôһɹݴѧ쵼ˮƽ
ʦʵġһ䷽ǰ˵Ļ̫ˣʲôѧУ쵼
¾ͻʲô˲š쵼ڰѧڰѧУ
ȫѧУҲ¼ȡʿʦ֪ʲôлɷ
Ȯ


Yhong888000ظڹ̳
http://geobbs.gywb.cn/bbs/viewthread.php?tid=66689

йרҵıƱҵڻеѧԺְܵ
ѧԺȥԺ  ܵģ͹ҵϵϲ  
㵱ԺѧҲˣҲ˵㣬
ѧԺ蹤ҵϵ   йҵϵģרҵ   
˵ѧԺҵϵĸڣ˶   רҵĸˣ
˭ оȴǻеѧԺ㲿Ƶо   д
ˣҪˣ ҵϵһϵ֪ѧԺйҵ
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֪ݴѧΪ˰ƽְ(   Բ  еѧԺĹҵרҵ
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㽭ѧѧϵ10ƪActa Crystal. (E)һоѧ

zjy

㽭ѧѧϵоѧʼ 涨һƪһSCI
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ЩڿԶܺͷƪActa Crystal. (E) ȣĽ϶Ƿ
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ʲôڿϾһֵļ򵥾ṹͶ彻Ǯܷ
ѵ2ƪActa Crystal. (E)ܺһƪJ.Org.Chem. Org. Lett.
оԹԡΪһ֪ѧźѧ
ӦýţһֹѧδѵһѧӦеΪ

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У֮Ҽ

ߣƥ

ĸУУһֱΪڸ˿ЦЦ
ڵ¸߹ͷ­ҲͬضԸУдڴҪ˵һ
˵Ĳͬ

ԸУе֮һǽѧ½Զ׼ģΪе
ǰ׼޷ѧѧУѧˮƽȴûӦߣ
ѧ½Ӧ˵"ѧ½"̶ܴǴӰٷֱ˵
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Ϊ95%ȴ40%½úܿ°ɣǴӾ
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˵ڵıҵҪУѾˣͨһУֻҪѧ
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ͼҳĳھͶĲЧСЩⲿѹʹ󲿷ѧԾ
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䣬ҺܻǵԾѧϰľͷѧôЩⲿѹ
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ֻ̽Ժʵ֤Ŀѧ

ߣJames

Ŀеǵĸɲ棬ô˼
òʾ͸ĸġ

ҸԸСͶԺܶصĶֲšЦ̬ȣѧʱ
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ҳעƼʽ



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һͨ˿ߣʶ˿ʼ죬Ҳɸͷˡ
˿̫ණѧ֪ʶϢɵ˼롣

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www.dajiajijin.org֮ʽһССĿע
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ˣԴҺܲԺҽÿʽ200Ԫǣ1
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3ش仯˿ͷĿգһ»Ч
ƷױƷҩϵǮҲֹĿˣֵõģ
ҲҿԳܵġ

ڶ̷۰֮ΪҾסһٳֵĹȶ
ŭͬʱ˼ԼΪı״һʲôٶʿǵĳ
Ŭ˿ɹͿΪԼܶŬԹۡ

ͬʱ˿Ѻ֪ĹˣǶعעƼ
ʽ𣬲ԼܼķΧڸ֧֣

һͨ˿
2008-10-11

(XYS20081012)

˿(www.xys.org)(xys2.dxiong.com)(www.xysforum.org)(xys-reader.org)

йαѧӪҪ壿

ѧ֪ͣͨ

һ磬пԺȻѧʷר̸ѧ
¡˵ѧԺ쵼йԺпԺ
֪ͨңѧҪȻѧʷٰ졣4ѷ
̶ͬ⣺1ѷ3773αٵĶ
ˣ1012գգ9-1130774̶
Ĺףˣ1019գգ9-1130776 ڶ
йӰ죨Զ1026գգ2-430ڱ
137ȻѧʷҾٰ졣2775Ǯѧɭѧԩ[
Ϲ]ƽ񴨣Բڰ졣

᳡б䣬վѧѶճ

ѧ֮ڱֵһּйƶ-
--󽻲ۺѧоͽڷչֺ
оƶԷԿѧлͳĻȻѧƶƼ
¡չĻԡƶѧչпѧȵȷϴ
Ŭ 21ʷչԽԽ֤ѧĹ

ģѧܼ18꣬쵽770йصλ͸
רҡѧߡѵĹĺֲַ֧ġҲҪлпԺȻѧʷ
Ŀݺ֧֡

ѧҪ֯ 
2008108

(XYS20081012)

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Ծ̳

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ˮİʳ߶ˣڸãڽ
Ʒӵ˼£ȿˮͺüأû൱ˮ
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ԲҪ200ˣܸȵģһҪԱ
̣ߣ̲̣˺ȵ̣80%ˮ
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˴ҵ飬ιȴƺһʮѵ£
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ϣܱ֤Լ˯ʱͲˡԾ̳йҽ
ִҽ֪ʶϵУȱ֪Ȱרҵ֪ʶҲ
רҵ֪ʶϵ֪ʶҽѧ֪ʶ

˿ϲμҽƷıۣûдҽרҵ
ϵۣȻǰҽѾ겻ҽӴҽѧרҵ֪ʶ
ˣһ֪֮ģҽѧרҵ⣬͵ò»߸ϰ
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һҾ̷Լӻʽҽר
ҵΪϵĺƣҵİˮ춣ϰҽѧרҵ֪ʶ
ǲΣҵдƻԭ

Ѿ㵱ҽˣȻҪҽѧרҵ϶ҽҽգȻ
Ҵ̣йҽʰԼļԣֻҵĲиļ
йҽûвϺٷ۵ϰߣ˼άϸǳ
˿ʶνִҵ⣬޲ǳ֪ʶȱ
ȫרҵѵȫҽѧ֪ʶǵ޲Եɡƫִ
Ц

ҽ۹УҲòϣйҽѧڿѧʿ
֪ʶȻҲΪѣ벻󻹵һ٣ٶظҽ
һӦ֪ͨʶҲᵽ˶԰ѵԱࡣλ
ǷǡһλҲ˱ҽڵҽ
ἰҩǡϻλΪҽ

λԾ̳ġǷǡҽȤѰ̼òò
⡱ѰĹ۵㣬ϧأϰߣȻǿЦġ
ԭļ򲡻ǷҪԺơXYS20081007

Ц֮һȱ߼ǷǡԭģżȻһβѪǸߡ
ҽһμѪǽȷҽ֪ͨ߸顣աһ
ԭҲ˵Ƿǡ˻޷ȷ򲡡ԭ򲡶
ֻϣȻ˸򲡡ҪסԺƣѵڡǷǡ
ġ׼ҪҽǷǡǣӦ
ҽӦҽָӡơ⾨
߼Ѷ

Ц֮ȮաǷǡҳģ޷֤Ѱ
϶ô֪ˣѰβϻԸϸ̵йҽ
ڿƼϣԭרҵЩ룬˵ʵڵģԸིҵ
ȷӦϺ֪ƺڿѧʿȻ࣬ǷǡҴһ
ǹҲ֪Ѿȷ򲡻ߣĿǰԭǣơ
ơۺơʳƺͺʵΪݲͬ
ҩơԼġ»ˡǷǡһЩе
ҵ۽飬޷Լ˸˻߲ˣStraw manڳϷ
ѡ

Ц֮ĶϰǷǡԡҽҪҽ۲족
۵㣬ˡȷ𰸡һͨ飬Ӳû˶׼Լ
ϵĹ۵ϵѪòҽǷǡӲǶ
ȷ֤ʵҵ˵ʵϣһѪǼѪѾ٣
ǷǡĵĸʱδѪǼǷԵģ֪ʱйҽ
ʲô˵˳ѡǷǡ һ䣬֯ҺɲҵĨü
ģûϰСʱҪйҽûҪ
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һԲĴǣ֢ͪж͸Է֢ͪ򲡻
Ϊ򲡵ļԲ֢ʽרҴʲôѽѵѰ˵Ǵģ
򲡱ˡǡԲΪѰ֪򲡵ļԲ֢۾
δÿ졣

˵Ϊ򲡣û֢״;ǡ򲡡
šǷǡԻû֢״ȷǲ򲡡
֢״ԣеٴ֢״Խȱ磬Ҳ˵ǡ
򲡡йЩ񾭴ִҽ˵ǡ֢״㶮ʲô
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򲡾ʲô͡Ҳб򲡵س̶ȣ
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Ц֮ģѧ档Ҫá׼Ҫʵעı׼
ҪôнҪôڲοн֪׼ʲô
ĻʲôĹѰ˵Һû±д׼ƫҪ
׼˵רҵ鷳

Ƿǡõġ׼Ƿǡûһ
򲡣ԺָƣҪҪˣйԴͲ࣬
˷ѣǰܼӡڴҲҽԺһ£öԺ
ҽʱ༸ۡ

Ҽǵҽ̷ܹǵĸSemmelweisԲ
˻ʱĹףǷϴֿԤͷŲɱ֡ʱҽ
ԺִҽԺǴƻ˲סԺǮҽ
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ϣˣתҽѧϣʹҽѧؼϴ裬Ѳԭϴˣ
ʱҽѧĽģʽҲò޿ӡ

ǱϾSemmelweisʱִҽԺѾԱ
ȵı磬гɱģ۲ƣʹִѧֶεĿƣԺ
ڸȾԾһսʹҽԺսڿԤʱ
ڣǲʧġйҽԺĳɱģʽԺڸȾ
⣬ȻʡʡʹйõҽԺⷽ¶Ժϸй
ĻߣȻֻรˡ

һӦǵйѪĽѵʲôѵйҸ밮̲Уй
ҽϵмΡ˵йѪվĹ涨ڸѪ
׺ŻõġִҽѧǰȫģǶҽԺҽϵƣ
ҲǼġҽԺƲԭвŽҽԺزԺôҽԺ
ǶǿĲԭļɢأȲѣйҽԺٽĺãΪ
ûò֤ԼЩ˲ԭԼֿܵЩǿĲԭô
ԸⱧҽ˼άҿɲȥԡ

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ҽɫѣһ˺űգûѰı£ô֪
йҽõĲǲԭԼ²ԭ

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ṩ֮裬ϸҪ򲡲˵һص㣬ǶԲԭ
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ԺڸȾԭڵķգҪңͨ˵ĵط
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ʡһµĺá

֪йĺҽǲǶ̫æˣ˲Ϊйҽ
ЩרǺάйҽЧԽԽ˲
ҽˣЩʲôɫѽᡢҵ˼ʹϵä
ԡпԭϸߣרҵ֪ʶҲ̶ãΪ
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롰ǷǡͬڵġŽҹꡱȻйҽû
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ҩЧûУЧ

ҽǱĵĿģһǼ򵥵Ӧһ¡Žҹꡱ⣬
νڽɼ˲㣬Ѹ˽ͣ
鷳Ѱ

˿һ꣬СڴҶзȲ⣬Ǻ
С÷ȡ˹˿ͣǵҪɣѡҲ
ȷвٵͿʼˣЧǣǻعרԿģ
⣬ҪŵϹӰꡱȥշѱ˺ô
ܿ

һǴģ˿ŷǵҪף˵Ҫ
ţһŵأһɳӣʵĽŵ棬ǲ
һɳӣȻλ˿Ϳɲô򷢵ģѽͣ鷳

ӵΪΪִҽѧҽҩгҩ
ֱӹйҽҵĶ⣬Žҹꡱ̹Ȼ˶⣬Լ
ܣͼʡ£ҲȱµذѻжĶ˻߿Уget back at you

ʼҽԱı޹ҽûרҵԻߵ
Ҫ󲻶нڵؾܾйҽĵλûָߣļֵ
ǲܵõϵġɳһ࣬Ҫ˿Ψһѡ
ͬѣ໥ͼҪҩõҽĬϣô
ǵĲͻؼҪҩйҽ
ʲôѡ

оҽѧʱҽ෴вٵ
ˣѡҽƣҸҽĽҽȫղ
飬ҽѧ뿼ΧڣûڽҽѧʱιȻõ
ܵŻΪʲô˺ҽ֪ڽҽѧƣ
йҽָܸܲһԽ

йҽĪе۵£ԼԼܵӣǾͶ
ٺЩ̬ζˡŽҹꡱҶòò̷ˣ
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ҽ޺ԭһءҵθУٴܣΪռȷ
ҽѧ֪ʶǱѭԭ

һҽþ֢ٴӦԷʱṩ֤
ѡҽѧдǿ֢ζҽȫãΪ
Ȼ˼άͣҽĲΣִҽѧδš

ǳԾ̳ģְҵĺãйеɲƲ
ҵαҪһաܿҵأ

(XYS20081012)

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˵˵Ҳߺš

ߣ

ΰdiǽĴȻδٷϢ֤ʵּ˴Ųǿ
Ѩ硣ӽйչٶٷչľǲɶҡ
ģΰdiǽĺճҲٷݵľҲǲɶҡ
ġֹڼҪĻѶȼ

ĿǰǽķչΪ׶Σһ׶ΣҲ
Ǿ̬վѼٷݲϿɵľվ
IPַֹڵЩϵķ
ʵ֣ҶͨӰ첻󡣵ڶ׶ΣҲĿǰĽ׶Σ
ʶ𣺾ʾWeb߾ʾڷ
ϢһضĳĶһ飬һν
дʡ˷ӣȻ󽫴˷롰
ֹʣƲ⣺ַʱƣҶҪ
ܻһ棬ֻǽֹµٶȷʴ˷
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ĿǰڸҵGoogleַΪˣԾյһЩʼ
вСдʡƷǰֻҪ򿪴ʼĿ¼
GmailӾͻжϣǳʱGmail
ܴ䣬Ͳٳ֣ԭڴˡ

ǽǻȫΪϵԭֻڵ
ھ⴫ʱܷãһԣֻضЭ飨
HTTPЭ飩ԣڹʹڡվٷһ
Ը취չڣССվϴڵҳ˵Ϣ
̺ûİͨҳҵȷ
ĳڵġĳϽվҳݲ
⣬͵ڲڡһ㣬ٷֻҪס棬Щ
վͷʲôˡгķչ𽥼
ΪļҷṩУֻҪס⼸ҷṩ̾´
ˣѶҲ󽵵͡ӼϽҪסЩṩֻ̣Ҫ
ݿɾĳЩݣڼͿˣʵ֣
ǰߣͨʶԼӰ졣һ棬
Ϊ棬ҲȡߣһЩϵֻߡйط桱
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ûв͸ǽڹԻݼܵߺһ
£֮ǽڼҲһЩõ©Ҫƹض
ܵļŬһֱڶʱ׿гЧ磬˿ΪԸ
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ôİ취磬ĳЩʶ̬ζȽŨվʼ
ͻַʼ˵ķСҶֲ
ʼʹģ෴ʼĵķʽȽ׵õϿɺͻ
ӭ

homeڡҲߺţ(XYS20081010)޷
˿ɵʻ㣩жϺ͡ΰǽԼʲô
šϵ󣬸пܵṩԼΪԲŻ
еԶе治Եûѧʵ顱
ƴͲϲֵǹĻйء

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谷ر׼ ʳƷҵʴ

ձ20081006

й谷ţ¼ͬʱ谷ȫ׼
ϳ߶ȲͬҲϱ仯ӴʳƷҵѶȣĳЩ
ȫֹĲƷȴͨļ顣

ζţ¼ĺĽ谷ܻʾʳ
Ʒ谷2.5ppm𽡿⡣Ǳʾ̽
谷ΪʳƷӼҲȷӤ䷽̷ʵİȫ׼
ΪĿǰѧԸʶӤӰʶڿհסܻʾ
δֱȾ䷽̷ۡ

ͬʱȫҺ͵ļܻӽƶ谷
׼ⷽĿհס̨ܵһΰ䲼ع涨ȫ׼
0.05ppm¡ݴˣ̨屾ٻȸ˾(Nestle SA)ĲƷ̨ܻ
֮ǰ˵ڲй½ȸƷз谷ɷݡȸʾ
ǵĲƷǰȫģ̨巢ֵ谷Ȼڵʡ

ܣӡʮйƷз谷ɱ˹
(Nabisco)°±ɺϣMars Inc.M&Mţɿͼɿ
8.5-945ppm֮䡣Ϻɱ˹ĸ˾ʳƷ(Kraft Foods 
Inc.)Դ˽ʾɣйͬһƷļû
⡣

ӡʳƷҩƷƻIndonesia Food and Drug Control Agencyķ
ʾʳƷҵбԹǲֵģⲢܼĽ
˵ӡ谷ִ֯(WHO)ָ׼Ǹ
ʳ׼ƶġWHOܷһƪ˵ȱй
谷Ӱ켰໥õݣı׼кܴ
ȷԡ

ҵ˵ܱŷҸı׼ܵʳƷ۸
ǺͲƷѷ̫˾ͼ(Khaled Rabbani)˵
ͶԱ溬Ľܱ׼ǽҲӰ죬Ĵ
ֲƷͨ⡣

۴ѧѧڳµï(Daniel Chan)֧ۺ̨ʵиϸ
谷Ʊ׼˵״ʳƷȫûʣ⽫
ʳƷĿѡΧزƷ۸Ӱ죬ǲòԸܶ
̶ȵķѡ

923հ䲼涨Ҫ3¶ͯڡڸŮʳƷ
谷ó1ppmȺʵ2.5ppmŷ˱׼й
ܻζţ¼Уʯׯ¹ųƷһӵ̷У
谷ĺߴ2,500ppm

һЩйƷҽĵȾƷӰ
ľߡĿǰһЩѶǧֲƷ˼⡣

谷ɷݵӤ׶䷽̷й4ͯ5ͯ
йƶұ׼Ƽվϵ֪ͨ
30ڼ2ppm谷İ취

ִŷ谷ȫ׼ĻΪ谷һ˵Գ
ǰȫģԳʵӰ컹֪֮١һǰڵĳʳ
Ʒз谷ɷݺ״ǹע

ʳƷҩ(FDA)Ʒ޶ҪٻصȨҪ
̽Ʒ¼ܡԴFDAۺʳйáǷ
󣬼ǡҸݵĹԱֵгۣѶ߷
档

̨ųŵ谷ȡ̡̬ȣ924ոòű
ʾ谷ʵŷ˱׼

Sky Canaves

(XYS20081012)

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Ĵũҵѧʿʦ̰ڵĹţ棩

йũҵѧҽѧԺŴĴũҵѧʿʦ̰
ڼ߷ƪ(Сһ͡¶)⣬
ֱǣ¶ǵоΪβǵס¶
ΪζԱο߸աķҹʡ(С)Ǻε
ǷظǷѧٺǷ֮ռָı
оɹΪ̰ڼ߷ڡйҽѧ
³Ϊһ̰ڼ߷ڡJournal of 
Microbiological MethodsӢĳΪ¶ƪµͨѶ
ͬΪ漰ƪµʱΪߡ
漰ͨѶҲͬ漰ʱҲΪ
ߡ

һ̰, , źһ, º, , , ¿, 
, ԬƼ, ТԾѼԸײRT-PCRⷽĽ. й
ҽѧ, 2007, 37 (01): 38-42.

¶Cheng Anchun, Wang Mingshu, Xin Hongyi, Zhu Dekang, Li 
Xinran, Chen Haijuen, Jia Renyong, Yang Miao. Development and 
application of a reverse transcriptase polymerase chain reaction to 
detect Chinese isolates of duck hepatitis virus type 1. Journal of 
Microbiological Methods (2008), doi: 10.1016/j.mimet.2008.07.018.

Miao Yang, Anchun Cheng, MingshuWanga, Hongyi Xinga. 
2008. Development and application of a one-step real-time Taqman 
RT-PCR assay for detection of Duck hepatitis virus type 1. Journal of 
Virological Methods 153, 55C60.

һ¶ǵоΪβǵף

¶ǼѪ1Ѽײ(duck hepatitis virus 1, 
DHV-1)RT-PCRĽӦáڸĵ۲֣ߡд
Based on the data analyzed from the 3D gene sequences (GenBank 
accession numbers: EF064886, EU106104, EF064886, DQ864514, and 
DQ864515), we established a specific RT-PCR assay for the detection of 
DHV-1. 仰53DжڸRT-PCRĽҪģȻ
ߡû֮صѷ3ƪеκһƪΪοף

ע53DӦΪ4̰ڼύEF064886EF064886
ͬظгû׿ɹο⣬3(EU106104, 
DQ864514, DQ864515)Ҽҵĺⶨģֱ֮ص£

(1) DQ864514йص: Ding, C., Zhang, D., 2007. Molecular 
analysis of duck hepatitis virus 1. Virology 361, 9C17 .

(2) DQ864515йص: , Ŵ. Ѽײ3
ĩеĿ¡ͷ. ѧ, 2007, 23(4):312C319. 

(3) EU106104йص: Wang, L., Pan, M., Fu, Y. and Zhang, 
D. 2008, Classification of duck hepatitis virus into three genotypes 
based on molecular evolutionary analysis. Virus Genes 37, 52C59.

¶ΪζԱο߸գ

¶ײԼ¶׵ĶӦ֣5
ƪе15߱¶ġߡգģʽԵһ
Ϊգڶֺ/Ϊ֡5ƪ׷ֱǣ

(1) Changsun, C., Seung, K.H., Chanhee, C., 2004. Development of 
nested RT-PCR for the detection of swine hepatitis Evirus in 
formalin-fixed, paraffin-embedded tissues and comparison with in situ 
hybridization. J. Virol. Methods 115, 67C71.
(2) Chun, H.T., Nick, J.K., Hsiang, J.T., 2007. Molecular analysis 
of duck hepatitis virus type 1 indicates that it should be assigned to 
a new genus. Virus Res. 123, 190C203.
(3) Dongyou, L., Mark, L.L., Frank,W.A., 2004a. Specific PCR 
identification of Pasteurella multocida based on putative 
transcriptional regulator genes. J. Virol. Methods 58, 263C267.
(4) Dongyou, L., Mark, L.L., Frank, W.A., 2004b. Specific PCR 
identification of Pasteurella multocida based on putative 
transcriptional regulator genes. J. Microbiol. Methods 58, 263C267.
(5) Dieter, V., Freddy, H., Katleen, H., 2007. Multiplex PCR assay 
for the detection of high
virulence rabbit Staphylococcus aureus strains. Vet. Microbiol. 121, 
368C372.

Ϊ֤ʵһ㣬׵ʵбȽϡ2004J. Virol. 
Methodsû58޷(3)ܾ(4)
Ļ(3)(4)Ϊظá4ƪе12߱
¶ġߡա(2)ڽIn press׶
Journal of Microbiological Methodsɾڡ¶ѽIn 
press׶Σǡߡ޸ĵĽӦǱHsiang-Jung 
TsaiֺĽ

ķҹʡ(С)Ǻε

¶УߡҹıСк͹ֱΪ
Beijing province, Chongqing province, Guangxi provinceҹļʡ
ʡ͹ʡֱΪJieling province, Yuennan province, gueizhou 
province 

̰ĴũҵѧĽںͲʿʦе˿Ƽũҵ
ĴʡĶشĿ(һ¶)ӳ̰ڻý
˲ּ֧ƻĿ̰ҹ
šӳ̰ڻĴʡܳѧƴͷ˻Ŀ
̰ĴʡܳѧƴͷˡӰٶȼ̰ڵļ
֪̰Ĵʡѧͼͷˡйҽѧᶯ΢̬ѧ
³йҽѧݲѧ᳣ֻ¡ѧίй
ҽ־ίйҩίԱίԱ˭Ҳ
ʶᷢһˡ㡱Ĵѧڡʿʦ
ѧƴͷϣȷʵˣ̰ǡ¶5еһ
֮һ2ͨѶ֮һʵһˣܽЩͼ
ķƵߡϡҲҪʣһʲôѧ̬ȣǷ
ԵҼίĴʡίЩشĿ

ġǷظ

һ͡¶ݾɷΪ֣һǼ1Ѽײ
(DHV-1DHV )RT-PCRĽǸRT-PCRӦá

Դڡһ͡¶һȫͬ
RT-PCRĽߣRT-PCRӦϣƪµ˼·
ͬ¶ûáһΪοס

(һ)	ƪмDHV-1 RT-PCRֵıȽ

1.һΪFP(5-ACAATGACCCAGCCTTAG-3)RP (5
-CCACTGTATCTTCCCTTC-3)¶ΪFP(5
-acaatgacccagccttag-3)RP( 5-ccactgtatcttcccttc-3)

2. İл򣺾ΪDHV-1440 bp3D

3 .RT-PCRϵͬһ1.5.1͡¶2.4

4. Լ⣺һȡյĺ飬Ѽ
ѼϸСϸСͲԳײв(H5N2)
ѼͷѪ֢ѼԴɱ԰ϸ˾(5A)ѼԴɳžѼԴ
˺ɳžѼԴ²Դ󳦸˾(O78)ѼҺѼΣ
¶飬duck plague virus (DPV) DNA, muscovy 
parvovirus (MPV) DNA, avian influenza virus (AIV) RNA, Pasteurella 
multocida (PA) DNA, Riemerella anatipestifer (RA) DNA, Salmonella 
enteritidis (SE) DNA

5. Լ⣺һУǿCHv-1ĺϵϡͣȡ15
ϡͶڼ⣬ʹÿ10 lзֱ𺬺31011C310-3 pgԽ
Ϊ30 pg¶УDHVĺϵϡͣȡ15ϡͶڼ⣬
ʹÿ10 lзֱ𺬺31011C310-3 pgԽΪ3 pg/10 l


() ƪмDHV-1 RT-PCRӦòֵıȽ

RT-PCRӦò֣Ȼһ͡¶д
ͬƷϸҲͬȽϣԿɼƪĸͬ
飬һ֤ʵĿɿԣóͬĽۣ
RT-PCRԻʸDot-ELISA/ELISAͲ롣һ
ʹõġٴͼġȷΪѼ׵19ݸβϡ
飬1Ҳ3

1RT-PCR19DHV-1м

1.ͬ: ͬɴ19DHV-1440-bp3D򣬼
һͼ3͡¶Fig.1

2.ͬ

(1)ƷͬһΪٴͼġȷΪѼ
19ݸβϣ¶Ǵҹ11provinces19Ұ
(field isolates)

(2)Ʒʱ䲻ͬһƷ198711200511£
¶Ʒ19852006ꡣ

(3)Ʒʽͬ(һ1.6.2͡¶ı1)
עЩƵԡһ19ƷΪCD871103NC880302
MY890203LS900104DY910405QS920809CQ930908YA940701CD950607
NC960808MY970903LS980605DY990302QS001203CQ011123YA021011
MY030519LS040716DY051106¶19ΪD1986NC1988
MY1989LS1991DY1990QS1992CQ1993YA1994GX1995HB1996
HLJ1997YN1998SC1999HND2000GD2001HN2002GZ2003LS2004
BJ2006

2RT-PCRDot-ELISA/ELISAͲַ˹ȾѼ
֯бȽϼ

1.ͬƵ

(1)ȾǿͬΪCHv-1ꡣ

(2)ⷽͬΪ롢ELISART-PCR֮ͬ,
һdot-ELISA¶ELISAߡ(Zhao et 
al,1991)ӦΪELISA

(3)ԸļͬһУɴ18ֻѼĸм
Խ(100%)¶Уɴ10ֻѼĸм⵽
(100%)

2. ͬ

(1)ѼƷֺͬһ20ֻõ1ӣҹ
Ѽ˹Ⱦ10ֻΪհ׶գ¶10ֻ1SPF Pekin 
duckling˹Ⱦ

(2)ѼʱʲͬһУ10ʱѼ18ֻ
¶УȾ47-90 hȫ

(3)֯ͬһУȡ18ֻѼġΡƢΡ
⣬Ҳؼ⣻¶Уȡ10ֻѼڼ⡣

3RT-PCRELISAͲַԱƷбȽϼ

1.ͬƵ

(1)ⷽͬΪ롢ELISART-PCR֮ͬ,
һdot-ELISA¶ELISA˴δףĺ
ĺο׷ΪELISA

(2)¶ͬΪ-20档

2.ͬ

(1)ƷͬһΪ198711200511ٴͼġ
ȷΪѼ׵19ݸβϣ¶ΪTwenty-two liver 
samples from ducklings infected with the DHV-1 CHv-1 strain between 
1986 and 2006 (1 sample/year; stored at ?20 C)

(2)вͬһУRT-PCRΪ18꣬Dot-ELISA
Ϊ10꣬Ϊ6ꣻ¶УRT-PCRΪ22꣬
ELISAΪ13꣬Ϊ11ꡣ

4RT-PCRELISAͲַٴƷбȽϼ

1.ͬƵ

(1) ⷽͬΪ롢ELISART-PCR֮ͬ,
һdot-ELISA¶ELISA˴δףĺ
ĺο׷ΪELISA.

(2)ӽһУΪ56.25%Dot-ELISA
Ϊ75.00%RT-PCRΪ91.70%¶УΪ
55.7%ELISAΪ69.2%RT-PCRΪ89.2%

2.ͬ:

(1)Ʒͬһ48ݣ¶185ݡ

(2)Ʒ˵ͬһΪѼٴ֢״Ͳ仯Ѽ
¶Ϊclinically suspected diseased liver samples of 
ducklings

(3)Ʒɼʱ䲻ͬһУ۲־Ϊ2000~2005꣬
Ϻͷ˵1987~2005ꣻ¶У3ʾΪ2006114
2008112գ2.7(iii)ֱΪ2006520081¡

(4)ƷɼͬһУƷĴ졢ݡ
ϡ㶫ɹź͸18ʡ(С)¶У
ƷSichuan provinceChongqing provinceGuangxi province
Beijing provinceJiangxi provinceHunanAnhuiHebei province
Jieling provinceHelongjiang provinceYuennan provinceHainan 
provinceGuangdong provinceHenan provinceGueizhou province

¶Ͷ2008229գûáһΪ
ףȻ2008121գ¶8е4
ͶJournal of Virological MethodsˡһΪοס
ۺϿǡ¶͡ͶڡԼ¶͡
һƳ̶ȣһ⣬ߡǷʶ¶
Ϊһظ֮ڡ¶вáһΪ
ףΪ˿Journal of Microbiological MethodsרҶظ
ע⣿

塢Ƿѧ

(һ) Ｐòο

һ͡¶(FP & RP)оͬ
ߡƪݵĲοǲͬġ

ڡһǰԲ֣ߡдʵоԱڹ
DHV ĿĻϣDHV CHv-1ǿȫ(ı
)ṹСRNA ֪ṹǳơ
ɹڱон˼DHV RT-PCRӲϺͷ
ֿɼDHV CHv-1ǿ3D׻СƵĲοС

һͶʱ2006921գߡȷʵⶨCHv-1ǿ
ȫУʱӦ2006921֮ǰݴ1

1ȥˣCHv-1ȫвⶨйص¡ıĸ
ûνı£ߡڡһгѲⶨ
DHV-1ǿCHv-1ȫǷΪڸǡߡ(FPRP)еʵ
Դ(Ƿ)

ڡ¶ĲϺͷ֣ߡƣõĲο
¼ΪEF064886С빫ݿɼ¼ΪEF064886ʵ
ϾǷSichuan440bp 3DУFPRPλڸ440bp 3D
еˣˣ440bp 3DFPRPСݴ
2

2ߡʵȷʵⶨCHv-1ǿȫУ
ҡ¶ҲʹǿCHv-1ڡ¶ʱΪβ
CHv-1Ļзһлͬ(EF064886)
һ͡¶Ƶȫͬߡȴݵǲͬ
ĲοУΪΣߡһΪFPRP
(EF064886)FPRPģ440 bp 3D(EF064886)
FPRPУôõģ

ڡһۺСᲿ֣ߡдԵĺ
PCRĻĿǰDHV еĲοϣδ
ӦPCRDHV оڻDHV CHv-1 ǿ
ȫеĻϣ˼DHV RT-PCR

ʵϣ2006921աߡһͶйҽѧ֮
ǰݿѾͷ˺Kim˲ⶨDHV-1 DRL-62(DQ219396)
R85952(DQ226541)У̨TsengȲⶨ03D(NC_008250)H
(DQ249300)5886(DQ249301)ĻУҿⶨC80(DQ864514)
2C-3ĩԼE-633ĩ(DQ864514) 
(http://www.ncbi.nlm.nih.gov/)ͬʱ20069³ɳٿй
ҽѧݲѧֻϣѱйDHV-1ͻṹо
չΪйҽѧݲѧ᳣ֻ£̰ѵ֪Ҳ
ߡ˵2006921Ͷʱδע⵽ݿе
DHV-1Уע⣬һ޻20061229գǰ
20061018գ̰ںλ¼ΪEF064886
Sichuan440 bp 3Dύݿ⣬ѵݿ
DHV𣿾ݴ3

3ݿ20067ѮͷDHV-1 DRL-62ꡢ
R85952ꡢ03DꡢH5886ȫСC80E63Ĳе£
ߡΪҪ۲˵ĿǰDHV еĲοϡ
仰ǷΪ˵乤ʵԺ״ԣԻȡйҽѧ
רҵΣ

ֵýһǡδӦPCRDHV о
仰2006718գҵѧƼΪһߵһƪ
Ͷѧøľ(5-ACAATGACCCAGCCTTAG-3)
(5-CCACTGTATCTTCC CTTC-3)һѼײ440bp 3D
RT-PCRȻʱƼδ󣬵ȻҪʳ̰
ڣǷΪѧƼĸӣǷƼԭе
ԼRT-PCRͬʱƼĸ20069·й
ҽѧݲѧֻļ(601ҳΪܣȥ)
ľǼѼײ440 bp 3DRT-PCRĽΪ
ҽѧݲѧ᳣ֻ£̰ѵû 

() Ѽ

һĲϺͷ֣ߡд1. 1. 2 ʵ鶯Ϊ1
ӣҹѼELISAѪDVH ԣѪDHV RT-PCR
Ϊԡ

ҵǣЩѼӽ˹ȾǿCHv-1ͨ
RT-PCR˹ȾǿCHv-1Ѽ֯м⣬ͨ
Dot-ELISAͲĽбȽϷRT-PCRԺ
ɿԡȻЩѼе˹ȾΪRT-PCR׼ġ
ݴ4

4ڡߡDHV RT-PCR֮ǰȷ1ӣ
ҹѼѪDHV ΪʱʹõRT-PCRĸ

¶Уߡ11SPFѼ߷ֳDHV-1ǿCHv-1
10ֻ1SPFѼ(SPF Pekin duckling)DHV-1ǿCHv-1˹Ⱦ
飬ݴ5

5ڷֳCHv-1SPFѼߺ˹ȾCHv-11SPF
ѼԺδ

() ڵӾ

ڡһͼ31987-2005ɼ-20汣19DHV-1ȾѼ
ĵӾһ֣¶Fig.11986-200619ĵ
ӾҲǼһ֡뵽⣺

6һͼ3Уν19ѼƷǷΪͬһ
Fig.1Уν19ꡱǷҲͬһ 

() 19440-bp 3DǷڱ

ڡ¶Ľֺ۲֣ߡRT-PCR
Ķ19˼⡣Щҹ11ʡ(С)
ʱԽ21(1986-2006꣬б1)ߡ˵ Sequencing of 
these RT-PCR fragments showed no genetic variation over different 
districts of China (data not shown). 

Ȼǵо(Wang, L., Pan, M., Fu, Y. and Zhang, D. 2008, 
Virus Genes 37, 52C59)DHVĸ440 bp 3Dͬͼ
18C22%ĺв죬Ѫ1(A)ڲͬ0C9%ĺ
в졣ǵоõгԼⶨ⣬д
֮ǰGenBankDHVȫе440 bp 3D֡˸֮440 bp 
3Dȫͬ⣬ڻٵв졣

ڡߡδڡ¶ʾнҲδύ
ݿ⣬ˣҲߡ˵ġno genetic variationǷ
ʾ19ȫͬǣ7

721ҹ11ͬʡ(С)19DHVΪ440 
bp 3DȫͬȫͬĿжܣǷ
ζšߡδⶨ19Уνno genetic variation
Դģ

() 2122˹Ⱦ

¶Уߡһ(2.7.ii)ַ-20
22˹ȾѼĸƷȻڲϺͷ2.7(ii)ֿɼ
22ݲǴ21(1986-2006)ÿCHv-1˹Ⱦȡ
(ߡעÿһ)ÿһݣֻ21Ʒһ
ĶģȻߡڽ(2)˵Ʒ1986-2007ġ
ȻʱһǴģʱζǡߡԼдģ
1986-2007ʵģϺͷ1986-2006
д

ʹʱ1986-2007꣬22䣬ÿ궼ͬһǿ(CHv-1)
˹Ⱦ飬ƵĺԺڣıҪж󣿾
ߡڡ¶ı1עǿCHv-11985ģ90
̰ڷĶƪ£δ̰ùǿ꣡ϼ
ڵļ֪̰19857±Ʊҵѧ1990
7˶ʿҵĴũҵѧ199010£20002Iowaѧ
ҽѧԺѧߣ199510½Ϊڡݴ8

8̰ڵȴ19862007ÿǿCHv-1гѼ˹
Ⱦ鲢ȡϵĿж

() ELISA

¶Уߡ10ֻ˹ȾSPFѼ3ַ
ıȽϼ⣬ELISA(Zhao et al., 1991)(2.7 i)
ߡZhao et al.(1991)ELISAƷеDHV-1

ڶ2122ȡ22˹ȾCHv-1Ѽ185ٴƷ
ȽϼУELISAⲿδ (2.7 iiiii)Լ
ĺĲοףΪߡZhao et al.(1991)ELISA

ȻZhao et al.(1991)ELISAһELISA֪
DHVԭаѼѪеDHVġݴ9

9. ߡZhao et al(1991)ļELISAȷ3
й217ݸƷǷDHV-1?

() ڡ뷨

ǡһǡ¶ߡȡ(virus 
isolation)һ˹ȾƷٴƷ˼⣬RT-PCR
ELISAļȽϡǣߡûнʱõʵ
Ҳû뵽ʱǸݺָ꽫ΪDHVرDHV-1ģ
ݴ10

10. 벡ʱߡʹ˺ʵʲôָ꽫
ΪԻԣνݡ롱Ϊ
DHVѪ1(DHV-1)

ڡһУߡҽ48ٴƷΪٴ֢״Ͳ
仯ѼķѼĲϣе֪ʶ뵽
ʱͨԽжΪDHVݴ˽ΪѪ1(DHV-1)
˷˼ġҹDHVѪ1ͣ
DHV()ݲʱ߻Ѽ߻ϸĲ
ǲܼѪ͵ġȻߡ48ٴƷⲿûȷָ
ָ뵽Ѫ1DHVµģǿΪ
һνġ56.25%ĲʡָǴ56.25%Ʒз뵽
Ѫ1DHVζܹͬѪDHVġ뷨뼫׻
ֻܣѪ1DHVġRT-PCRмʻԵıȽϣ
ˣΪ56.25%Ĳһݡ

ڡ¶Уߡ185ٴƷΪclinically 
suspected diseased liver samples of ducklingsһģɵ
ֲϣ֪ߡ϶뵽ĲDHVһ
DHVѪ1ͣߡvirus isolationͽ103
ƷеķΪDHV-1໳ɡ¶3е55.7%Ĳ
һαݡ

ƪжٴƷĽҽиΪ꾡ķ

() DHVйƪ

1. DHVѪ

ȻѼײ(duck hepatitis virus, DHV)Ϊ3ͣ
Ѫ2ͺ3ֲͬ״(Http://www.picornaviridae.com)
ͳѪ2ͺ3ų󣬽оDHVСRNAһ
ĳԱΪѪ1(DHV-1)̨ͺͺ (Ding and Zhang, 
2007, Virology, 361, 9C17; Tseng et al., 2007, Virus research, 123, 
190C203; Kim et al., 2006, Journal of General Virology, 87, 3307C3316; 
Tseng and Tsai, 2007, virus research, 126, 19C23; Kim et al., 2007, 
Arch. Virol., 152, 2059C2072)̨ͺͺ֮Ѫѧϵδ
бȽϣǾDHV-1֮ûнкͷӦڻĸ(
3UTR)̨͡ͺDHV-1ಡ֮ڽϴĺв
죬ݴ˿Ʋ⣬̨ͺͺͿѪ͡

2. DHVĻ

VP1, VP0, VP3, 440-bp 3D(Wang et al., 2008, Virus 
Genes, 37, 52C59)Լ5UTRĲ(Fu et al., Vet Microbiol. 2008. 
131, 247C257)DHVɷΪ3ͣǽΪA͡BͺCͣ
ֱӦDHVѪ1(DHV-1)̨ͺͺ͡

3. ҹDHVѪ()״

ǵоҹһЩѼͬʱѪ1(A)
(C) (Fu et al., Vet Microbiol. 2008. 131, 247C257)
վ(йҽƼ2002, 32, 15C16)ͻ÷(㽭ũҵѧ
2007, 3, 343C345)ֱѪѧDHVĴڡʵ
20071219GenBankύ˻CͶC-GYĻ(Pan,M., Fu,Y., 
Wang,X., Xu,Y. and Zhang,D.¼EU352805ε,Ѽײ
3ĩ˵ص. йũҵѧѧ, 2008, 13(3), 65-70)ѧ
Ҳⶨ˼ͶĻУB63(GenBank¼EU747874й
ũWu, P.F., Zhang, G.Z., Su, J.L. and Han, B.ύ)G(GenBank¼
EU755009ũԺShi, S., Chen, Z., Yang, W. and Huang, Y.ύ)
FS(GenBank¼EU877916ũHe, R.Y., Zhang, G.H., Luo, Y.J., 
Sun, W. and He, Y.M.ύ)һ֤ʵͼCDHVҹĴ
ڡ

4. 440-bp 3Dе

(5-ACAATGACCCAGCCTTAG5-CCACTGTATCTTCCCTTC)״Ӳͬ
DHV-1440-bp3D(ָйũҵѧ2004˶ʿ
˶ʿѧλ)øﲻܴӻCͶC-GY440-bp 3D
(εȣѱйҽ־ܵĸ)

бȽϽʾ(5-ACAATGACCCAGCCTTAG5
-CCACTGTATCTTCCCTTC)C-GYĽΪԿԴC-GY
5Ĳ졣

й½δ뵽̨(B)DHVˣҲ
(5-ACAATGACCCAGCCTTAG5-CCACTGTATCTTCCCTTC)ǷɴӻBDHV
440-bp 3DСбȽϽʾѶȿܽϴΪ
BͶ90D04GҲ5Ĳ졣

֪ʶ(5-ACAATGACCCAGCCTTAG5-CCACTGTATCTTC 
CCTTC)DHVٴƷ440-bp3DУǿΪ÷
ٴƷѪ1DHVҲ˵440-bp 3DеRT-PCR
DHVѪ1ԡȻһRT-PCRǷһͲܴ
CͶ440-bp3DУCͶB63
ֻ3Ĳ졣ҵĹ۵ǣǽDHVRT-PCR
ǽѪ()RT-PCRFPRPѡ

и飺20059ָ˶ʿʼⶨDHV-1 C80ȫ
ʱݿûDHVͷţǰڵоǴDHV-1 C80
һ1246-bpУΪ֤DHVУ
(5-ACAATGACCCAGCCTTAG5-CCACTGTATCTTCCCTTC)ּͨʵ
ұDHV֤1246-bpȷʵDHV C80(йũҵѧ
2004˶ʿ˶ʿѧλ)ЧĴʵұļ
DHVѼƷ440 bp 3DУйũҵѧ2006챾
ƼʵҵʵϰʱҰøｨ˼Ѽײ
RT-PCRͶѧ()

ĿǰֻΪDHVٴƷ440-bp3D
У÷ٴƷһѪ1DHV(DHV-1)򣬡һ
RT-PCR48ٴƷʱõ91.7%Լ¶RT-PCR
185ٴƷʱõ89.2%ʶǴġΪӡһ
¶ĿɿߡǼDHV-1RT-PCRĽ(Ӧ
)

() ڡһRT-PCRͲ뷨ĽȽ

һ48ٴƷ4ΪRT-PCRԡ44ΪRT-PCR
ԣ21Ϊԡ27ΪԣݡߡۺСᲿ
ֶԼĽһԿ4RT-PCRΪԵĲͬʱҲ
벻17ݷ벻ƷRT-PCRΪԣںߣ
ߡĽǡPCRԶ(Dot-ELISA)ԣ
Ʒʱ򷽷ԵͲܼ΢
йء 

Ϊֽûеġɴ¼

(1) ݡߡڲϺͷеĽܣѼٴ֢״Ͳ仯Ѽ
ȡеѼԸ׷֢״Ͳ仯ĸಡϣӦ
DHVšߡҲͬһ㡣

(2) ߡ걣ľȷΪѼ׵19ٴͼƷ
ļ֪DHV ĸ֯-20汣18󻹿RT-PCR
10Dot-ELISA6ɷ뵽DHV 񡣶48ٴƷȡ
20002005(ܡߡڲϺͷֳƲϲɼʱΪ1987-2005꣬
ڽֺСᲿ֣ߡƲϲɼʱΪ20002005꣬
ΪϲɼʱӦΪ20002005)ֻ16ıڣϴ
-20棬ֻҪкѼײӦǴġ

(3) ֪DHV-1Ǽ׷뵽Ĳǣһ48ݲ
У21ݲϷ벻ܣΪPCR
44ݲϿ϶Ѫ1DHVȻߡе17ݲδܷ
DHV-1ģ

(4) ߡRT-PCRΪ30 pgеķȥ
ѼDHVȴ4ݲĿߡԴ
δ͡

ߡõ48Ʒʵģٴ֢״Ͳ仯
ѼķѼضѼײҵĹ۵ǣЩƷ
ԸȾDHV-1ѼȺӦ440-bp3DУ
һ4PCRԵĲϣӦDHVǺл
CDHV򣬷ѼֳܱѼٴ֢״Ͳ仯֮
4ݲϵPCRԣΪԴڸ÷Ըߵͣ
ǡߡõDHVв졣

Ƿѻ߲ܷ뵽DHVشǷ񶨵ġΪ̨ѧѷ
BͶ90D04G(Tseng and Tsai, 2007, virus research, 126, 19C
23)ѧѷ뵽CͶAP-03337, AP-04009, AP-04114 
AP-04203 (Kim et al., 2007, Arch. Virol., 152, 2059C2072), ʵ
Ҳ׵ķ뵽CͶC-GY, C-YDF, C-BLZC-YCW (Fu et al., Vet 
Microbiol. 2008. 131, 247C257), оҲѷ뵽CͶ
B63ꡢGFSꡣ

ҵĹ۵ǣ48ٴ֢״Ͳ仯Ѽ
ѼĲ࣬44ݲPCRǿܵģ17ݲϷ
DHV-1Ľģ4ݲPCRԵĽǿܵģ
Ľġݴ11

111~6ڣȡٴ֢״Ͳ仯ѼķѼ48
ٴƷУ4ݵRT-PCRͲΪԣԭڣ
ǷζڲڲȾ£ѼҲܷѼԸףRT-PCR
ΪԵ44ƷУȷDHV-1Ϊλ17Ϊԣ
Ʒ1~6꣬ξû᣿ĺ(RNA)
δӲģǷή⣿ȡٴ֢״Ͳ仯Ѽ
ķѼٴƷУνǡ΢׼Ѫ1
ĸRT-PCRܼⲻͬѪDHVĲ뷨ǷԵı
ϣDot-ELISAУͬѪDHVǷڽ淴Ӧ϶Ĳһ
Ѫ1DHV? ߡǷΪı조½RT-PCRһҪ
еDot-ELISAͲ뷨ԡһۣ

(ʮ) ڡ¶RT-PCRͲĽȽ

¶185ٴƷ20ΪRT-PCRԡ165ΪRT-PCR
ԣ82Ϊԡ103Ϊԡûһ
ԡ¶ļһϸǣͨԡ¶
3ķɽΪ2顣

1120ݸƷRT-PCRΪԣvirus isolationΪ
65.8% (79/120)

265ƷRT-PCRΪ69.2% (45/65) virus isolation
Ϊ41.5% (27/65)

1. 1ķ

ͨ£ӸƷ440-bp3DУΪƷд
DHV-1ȻRT-PCRΪԵ120ƷУ41δܷ뵽DHV-1
ߡɴ˵óRT-PCRԸڲĽۡΪ
̫͡ݡݲѧOIEֲĽԼƽʱо
֪Ƚּ߻Ѽ߼׷뵽DHV-1رǣٴƷ
ȡ2006-2008꣬ʵĲϡʵ3ݲ2008112
ʡѼ1롰ߡ¶ͶJournal of Microbiological 
MethodsԼ1ʱ䣬Ȼ3ݲ1δܷ뵽ֵע
ǣߡ-20汣11ܷ뵽DHV-1ˣ
Ϊ65.8%ĲһĽ

Ҳߡ185ƷΪclinically suspected diseased 
liver samples of ducklingsʲôǣ185
ƷĲɼ޷ܡһֿǣɼĲ()Ѽ࣬
ƷпܺѼײвѼȡݴ
12

12ߡռ185ٴƷʱǷѼԸ׵ص
йٴϣclinically suspected diseased liver samples of 
ducklingsǺ⣿ѼѼǷֵ͵ѼԸ׵ٴ֢״
/Ѫ䣿յģߡ֪ѼǷѼ
Ը׵ٴֺͲ仯ߡνݡvirus isolation
һ120RT-PCRƷе79Լ185Ʒе103еķ
ΪDHVرDHV-1ģ

ڶֿǣߡڲɼ185ٴƷʱݲѼѼ
ٴ֢״/͵ĸѪгϡˣǽһ
2ݡ

2. 2ķ

ȻߡδԽϸĽܣܿRT-PCRΪ
Ե45ƷУвƷ벻DHV-1ǰġ

20RT-PCRΪԵƷԣ֪ߡνһ
ҲߡΪRT-PCRʲӦ100%ǰ2
65ݸƷǴӸȾDHV-1ѼȺɼģ()Ѽĸбض
㹻ĲɹRT-PCRע⣬¶RT-PCRļΪ
3 pg/10 lȻҹµѪ纫(C
)ڲٴƷΪRT-PCRԣǿģҲΪ뷽
ԸߵйأԴDHVв¡
Ҳ˵RT-PCRΪԵ20ݸƷУܺDHV
ĿǺк(C)

ڡߡδԼϸܣвRT-PCRԵ20
Ʒз벡ĽΣ޷3ֿԡ 

(A) 20Ʒо벻 

һġǰRT-PCRΪԵ20
ݸƷУܺDHVк͵ĿǷѻ
ܷ뵽DHVشǷ񶨵ġǰ

Ҳߡ˵ʹ뵽DHVΪǲѪ1ͣҲֻܽ
¼Ϊԡǣݡvirus isolationͿɼѪ

(B) 20Ʒоɷ뵽 

RT-PCRΪԵ20ݸƷ뵽Ϊȷġ
ǣǼ¼ΪΪԣΪǴġΪߡ
˵ĲָǷ뵽Ѫ1DHVڸRT-PCRԵƷ
з뵽ضDHV-1ĳDHVˣһ
Ҳġ
(C) 20ƷУֿɷ뵽ַ벻

(A)(B)ϡ

Ϊ185ݱΪclinically suspected diseased 
liver samples of ducklingsƷߡյģ89.2%
RT-PCRǹߵģ55.7%ĲٵġߡԷ
Ѽ˳ϣĿռе͵ĸѪĲϣ
89.2%RT-PCRǿܵģ55.7%ĲġPCR
Ե20ݲϣ۲ĽΣġ
1314

13ѼѼֵ͵ѼԸ׵ٴ֢״/
Ѫ䣬1~3ռ185ݲУΪ82ݷ벻
120ݾRT-PCRΪԼ϶DHV-1ƷУΪλ41ݷ벻
DHV-1? 

14ѼѼֵ͵ѼԸ׵ٴ֢״/
Ѫ䣬Ϊ20ƷRT-PCRΪԣRT-PCRΪԵ20
ƷУмݷ벻ǷζڲڲȾ£ѼҲ
ѼԸףмܷ뵽뵽ʲôΪʲô
RT-PCRΪԵƷҲܷ뵽ξݴ˵óRT-PCRԸ
ڲ롱һۣ

⣬ڡһ͡¶УߡԱĺƷ
ַ˱Ƚϼ⡣һĺౣ18(198711
200511)18󻹿RT-PCR10Dot-ELISA
6ɷ뵽DHV 񡣡¶ĺౣ21(19862006)22
(19862007)22RT-PCRELISAͲֻ
Ᵽ1311DHV-1ݴ15

15ƪУDot-ELISA/ELISAͲļ޴35
Ĳ죬Ϊʲô

󣬽ϡһ¶͡ǿԿڡ
һߡCHv-1ȫɲ(ǰԲ)ö440 bp 
3DҲѲ()ڡ¶ߡƲⶨ19
440 bp 3DУڡߡƲⶨXC440 bp 3D
СȻƪ£бȶͼߡҲûн
Щύݿ⡣һ棬ߡڡ¶͡о
ʹҿⶨ(DQ864514, DQ864515, EU106104)Ϊ
RT-PCRԼӫPCRƵĲοڡУߡ
ƣӫPCRʱʹõ(P3P4)̽(FP)ǻ3е
440 bp 3Dкⶨ440 bp 3D(EF064886)Ƶģ
ʵϣ4440 bp 3Dȫͬݴ1617

16ߡѲⶨЩǷʵģ

17δ4ȫͬ440 bp 3DѡӫPCR
̽룿

ǷԵ

2006괺йũҵѧӦ챾ƱҵƼʵҽбҵʵϰ
ָ2004˶ʿоһЩ顣Ƽд
һƪ£ΪѼײRT-PCRĽ20067
18Ͷѧ(60108ߣƼŴͨѶߣ
Ŵ)Լ3µڣ±ܡ

2006921գһ̰ڵͶйҽѧ
2007귢

ȽƼͶѧָ͡һɷƼָ
һͬͼƵݡ

(һ) RT-PCRõȫͬİлΪѼײ
3D440 bp

(1) ƼеƲ ݱʵǰڲⶨDHV C80
(DQ864514)Primer premier 5.0F4405 
ACAATGACCCAGCCTTAG 3R4405CCACTGTATCTTCCCTTC 3
Ϻ﹤̼޹˾ϳɡ

(2) һƲ DHV CHV-1 ǿ3D׻
УPrimer Premier 5. 0 FP5
-ACAATGACCCAGCCTTAG-3䣬RP5-CCACTGTATCTTCCCTTC-3䡣
ɴ﹤޹˾ϳɡ

лѡƺͲȡǽRT-PCR
ؼ衣ڷѧоչԽѼײԣ
ǽRT-PCRⷽĺģر20067֮ǰһDHV
ĻУȻ̨ѧߺͺѧʱⶨ˼DHVĻвύ
GenBankûͷţʱжڽѼײRT-PCRⷽ
ҪǲԶģ

() ȡ֣ԼԼļҲ
Ϊơ

(1) Ƽеĺȡ Һ10000 r/min5 min
ȡ塣֯0.3 g1:5ˮĥ3Σ8000 r/min 
10 minȡ塣Һ50 leppendorfܣμ200 lѽ
Һ5 lǴ (1 mM)Ծ۱ϩ (20 g/)ߵȺ
10 min2ԤˮҴȺ-20澲5 min12000 
r/min 8 minҺ70Ҵϴӳ12000 r/min 2 min
Һĳܽ30 lRNaseøȾˮСͬ
ҺͽѼĸȡΪա

ڡƼRT-PCRֻᵽԢDHV C80Ϊ飬Ѽ
ѼϸСϸС츱ճ²
ҺͽѼ֯Ϊ飬RT-PCR

(2) һĲĳᲿ DHV ǿCHV-1 Ѽ
Һ8 000 r/ min 10 minȡ壻ȡ֯0. 5 g110
ˮĥ3Σ10 000 r/ min 15minȡҺҺ50 
L EPУμ200 LѽҺ5 LǴ(1mmol/L)
Ծ۱ϩ(20 g/)ߵȺ¾10 min2Ԥ
ˮҴȺ-15澲10 min13 000 r/ min10 minҺ
700 mL/LҴϴӳ13 000 r/ min3 minҺĳ
ܽ30 LRNAøȾˮСͬȡѼҺѼ
ΡѼѼϸСϸСͲԳײ
( H5N2 )ѼͷѪ֢еĺΪջԼ顣

ƪûб˴ԣ˫ͬʱӳΪ1359 nt3Dѡ743
1182λΪл򣬲ͬеͬķȡ
ᣬüͬȡ̣ɺϵĸ
ʵжɺ϶£Ƽıܸˡ
һǡһƼıܸ壿

ұ֤̰˵ġһ2007깫֮ǰǴδ
̰Ƿܱ֤2006718(ƼͶѧ)2006
921(һͶ)֮δƼĸΪѧ
ıί̰ǷƼĸӣǷ֮ʲµĽ
ƼеݾΪУ

һ͡¶õǡߡԭģ
⣺

(1) һ͡¶УߡһȫͬRT-PCR
ڡһߡFP(5-ACAATGACCCAGCCTTAG-3)RP(5
-CCACTGTATCTTCC CTTC-3)ǻȫѲⶨCHv-13DƵġ
¶УߡFP(5-acaatgacccagccttag-3)RP(5
-ccactgtatcttcccttc-3)ǻڵ¼ΪEF064886440 bp 3DƵġ
Ϊʲô

(2) ӡߡѲⶨCHv-1ȫȥˣߡ
CHv-1ȫйص¡ıĸ

(3) ¶У¼ΪEF064886оǷSichuan440bp 
3DУFPRPλڸ440 bp 3Dеˣˣ440 bp 
3DFPRPС440bp 3DоFPRP
Уθһ440bp 
3DвFPRPУô440bp 3Dʲôֶε
ģǷǡߡύGenBankеC80E63нȡ
ģ

(4) 2008121գ ¶8ߡе4һƪͶ
Journal of Virological MethodsѷΪMiao Yang, 
Anchun Cheng, Mingshu Wang, Hongyi Xing.2008. Development and 
application of a one-step real-time Taqman RT-PCR assay for detection 
of Duck hepatitis virus type1. Journal of Virological Methods, 153, 55
C60

ڡ2.2.1֣ߡдAccording to the sequence 
of DHV-1 isolate Sichuan obtained by the Avian Disease Research Center 
of Sichuan Agricultural University (GenBank accession no. EF064886), a 
conventional RT-PCR was carried out using a template from DHV-1 XC 
Strain, with primers P1 and P2 (generated by TakaRa, Japan) targeting 
the conserved region in the 3D gene (Cheng et al., 2007). The product 
size was 440 bp עο(Cheng et al., 2007)ǡһ

˷ѽ⡣ߡû˵P1P2Ǹο
Ƶģȴ˵ԡһ3DıΪͬʱһͳ
RT-PCRǸDHV-1 Sichuan(¼ΪEF064886)ʵ
Ҳǰ440 bp 3DС

ȻߡڸδгУݸöϢ(P1P2
DHV-1440 bp 3D)ͽֵһϢ(øDHV-1 
XC440 bp 3D¼ΪEF064886440 bp 3Dȫͬ)
ҲжϳP1P2ضλ440 bp 3DеˡP1
P2롰һ͡¶õFPRPDHV-1ͬ
440 bp 3DУرǡ¶СߡͬǸDHV-1 Sichuan
440 bp3D(EF064886)FPRPǷP1P2FPRP 

ڡ(P1P2)͡һ(FPRP)ͬ
440 bp 3DУҡˡһΪο
ףʱ⣺

(i) ΪΡߡڡвֱʹáһе(FPRP)
ȴʹһԲе( P1P2)? 

(ii) һǰУߡֻCHv-1ĻѲⶨ
һĽ֣ߡҲֻƲⶨCHv-1440 bp 3D
(ȻCHv-1ȫк͸ö440 bp 3Dǿ)
ƪûʹκ3DнзȽ(ǰߡ
ΪʱûDHV-1еĲο)ôֻһ
(CHv-1)3Dе£е(P1P2)ΰ3D
ıģ

(iii)ġDHV-1 Sichuan440 bp3DУһ
ͳRT-PCR鱻ɡʲô˼ǷָݸP1P2
ˣΪʲôP1P2ҪһCHv-13Dеı
ȫѲCHv-13DУΪλҪDHV-1 Sichuan440 bp3D
һΪP1P2лP1P2 

һ˵ǣ֤ʵһġߡԣǾζţ
Ĵũҵѧʿʦ̰ڼӵὫйũҵѧ
ƼָеݾΪУڲһЩΪαݺ󣬼
ʵظڡйҽѧ͡Journal of Microbiological Methods
һУڡоȹ18˴β룡Ҳζţڲ
ʱڣ2006718(ƼͶѧ)2006921
(һͶ)֮䣬һġߡ18ֻ˹ȾѼġ
ΡƢΡԣ10ֻѼġΡƢΡԣ19걣Ʒ
48ٴϹ207ƷRT-PCR⡢Dot-ELISAͲ룡
һܵĲԽ㣬ߡӦ뵽104꣬
ͬʱߡݡ롱һ뵽104겡ΪDHV
Ѫ1ͣǷܣܣһ֤ʵһѧ
Ϊ

йũҵѧҽѧԺ Ŵ

2008109

(XYS20081012)

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